ELAVL1 is a general regulator involved in TNF-mediated cell death. (A) HeLa cells (parental, ELAVL1 KO clones #1 and #2 with different gRNA) were treated with IFNγ for 24 h (n = 3). The mRNA levels of CYLD were measured by qPCR. One-way ANOVA was performed: NS. (B) HCT-116 cells (parental, ELAVL1 KO clones #1 and #2 with different gRNA) were treated with IFNγ for 24 h. The expression of indicated proteins was analyzed by immunoblotting (n = 3). (C and D) HeLa (C) or HCT-116 (D) cells (parental, ELAVL1 KO clones #1 and #2 with different gRNA) were treated with ABT737/S63845 for 12 h (n = 3). Cell viability was determined by ATP levels. One-way ANOVA was performed. (E and F) HeLa cells (parental, ELAVL1 KO, ELAVL1 KO stably expressed with WT ELAVL1 or mutated ELAVL1) were treated with TNF/Smac (E) or ABT737/S63845 (F) (n = 3). Cell viability was determined by ATP levels. One-way ANOVA was performed: **P = 0.0053, ****P < 0.0001 (E); NS (F). (G) The mRNA levels of indicated genes involved in TNF signaling were measured in HCT-116 cells (parental, ELAVL1 KO clones #1 and #2 with different gRNA) by qPCR (n = 3). One-way ANOVA was performed: ****P < 0.0001. Data represent the mean value ± SD from n biological replicates or a representative immunoblot from n independent experiments. Source data are available for this figure: SourceData FS5.