IFNγ induces CYLD and caspase-8 expression in murine cell lines. (A, E, and I) BMDMs (A), EMT6 (E), and B16F0 (I) cells were treated with IFNγ, TNF, or TNF/IFNγ for 24 h. The protein expression of CYLD, caspase-8, and iNOS was analyzed by immunoblotting (n = 3). (B–D) BMDM cells were treated with IFNγ, TNF, or TNF/IFNγ for 24 h (n = 3). The mRNA expression of Cyld (B), Casp8 (C), and Nos2 (D) was measured by qPCR. One-way ANOVA was performed: ****P < 0.0001 (B); **P = 0.0029, ****P < 0.0001 (C); ****P < 0.0001 (D). (F–H) EMT6 cells were treated with IFNγ, TNF, or TNF/IFNγ for 24 h (n = 3). The mRNA levels of Cyld (F), Casp8 (G), and Nos2 (H) were measured by qPCR. One-way ANOVA was performed: ****P < 0.0001 (F); ***P = 0.0003, ****P < 0.0001 (G); ****P < 0.0001 (H). (J–L) B16F0 cells were treated with IFNγ, TNF, or TNF/IFNγ for 24 h (n = 3). The mRNA expression of Cyld (J), Casp8 (K), and Nos2 (L) were measured by qPCR. One-way ANOVA was performed: ****P < 0.0001 (J); *P = 0.03, ****P < 0.0001 (K); ****P < 0.0001 (L). Data represent the mean value ± SD from n biological replicates or a representative immunoblot from n independent experiments. Source data are available for this figure: SourceData FS2.