Figure 2.
Sister chromatids are separated during DNA combing but not spreading. (A) Representative images of DNA fibers obtained with the combing approach. Scale bar is 10 µm. (B–D) DNA combing assay performed as depicted in Fig. 1 A. Green-only versus red–green or red–green–red tracts are scored and represented as percentage of total pulse-labeled tracts. At least 100 tracts were scored for each sample. (B–D) Independent data sets from the Vindigni, Caldecott, and Chaudhuri laboratories, respectively. N indicates the number of biological repeats (N = 3 in B, N = 6 in C, and N = 3 in D). Statistics: mean ± SEM. Numbers indicate the mean tracts %. (E) Representative images of DNA fibers obtained with the spreading approach. Scale bar is 10 µm. (F) DNA spreading assay performed by the Vindigni laboratory as depicted in Fig. 1 A. Green-only versus red–green or red–green–red tracts are scored and represented as percentage of total pulse-labeled tracts. At least 100 tracts were scored for each sample. Number of biological repeats N = 3. Statistics: mean ± SEM. Numbers indicate the mean tracts %. (G) Representative images of DNA fibers obtained with the hybrid approach where the DNA is spread on the coverslips using the combining machine. Scale bar is 10 µm. (H) DNA spreading with combing machine assay performed by the Chaudhuri laboratory as depicted in Fig. 1 A. Green-only versus red–green or red–green–red tracts are scored and represented as percentage of total pulse-labeled tracts. At least 100 tracts were scored for each sample. Number of biological repeats N = 3. Statistics: mean ± SEM.

Sister chromatids are separated during DNA combing but not spreading. (A) Representative images of DNA fibers obtained with the combing approach. Scale bar is 10 µm. (B–D) DNA combing assay performed as depicted in Fig. 1 A. Green-only versus red–green or red–green–red tracts are scored and represented as percentage of total pulse-labeled tracts. At least 100 tracts were scored for each sample. (B–D) Independent data sets from the Vindigni, Caldecott, and Chaudhuri laboratories, respectively. N indicates the number of biological repeats (N = 3 in B, N = 6 in C, and N = 3 in D). Statistics: mean ± SEM. Numbers indicate the mean tracts %. (E) Representative images of DNA fibers obtained with the spreading approach. Scale bar is 10 µm. (F) DNA spreading assay performed by the Vindigni laboratory as depicted in Fig. 1 A. Green-only versus red–green or red–green–red tracts are scored and represented as percentage of total pulse-labeled tracts. At least 100 tracts were scored for each sample. Number of biological repeats N = 3. Statistics: mean ± SEM. Numbers indicate the mean tracts %. (G) Representative images of DNA fibers obtained with the hybrid approach where the DNA is spread on the coverslips using the combining machine. Scale bar is 10 µm. (H) DNA spreading with combing machine assay performed by the Chaudhuri laboratory as depicted in Fig. 1 A. Green-only versus red–green or red–green–red tracts are scored and represented as percentage of total pulse-labeled tracts. At least 100 tracts were scored for each sample. Number of biological repeats N = 3. Statistics: mean ± SEM.

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