Treatment with G3Ia prevents the formation of stress granules and dissolves pre-formed stress granules in human iPSC-derived neurons. (A) Schematic showing the paradigm used in B and C. iPSC-derived cortical neurons were treated with 50 μM G3Ia or G3Ia′ in the presence or absence of 500 μM NaAsO₂ for 60 min followed by fixation and imaging. (B) Representative images showing stress granules via staining of G3BP1 in cortical neurons in the presence or absence of G3I compounds under baseline or stressed conditions. Scale bars, 20 and 5 μm (inset). (C) Quantification of cells as in B showing the percentage of cells with stress granules under each condition. Error bars represent mean ± SEM. (D) Schematic showing G3BP1-mNeonGreen iPSC-derived neurons exposed to 500 μM NaAsO₂ for 30 min, at which point indicated 50 μM G3I compounds were added. Shown are representative images showing live cell imaging of stress granules via mNeonGreen-tagged G3BP1. Scale bars, 10 μm.