G3I compounds do not alter translation under basal conditions or following sodium arsenite stress. (A) Schematic showing the preincubation paradigm used for pretreatment with 50 μM of indicated G3I compound or vehicle control. Indicated doses of the compound were added to HeLa cells for 15 min, followed by exposure to 500 μM NaAsO₂ stress for 30 min. Puromycin (500 μM) was added to the media 15 min following the addition of NaAsO₂. Unstressed cells were used to quantify the basal translation rate. (B) Cells were collected and lysed with RIPA buffer followed by SDS-PAGE. Newly synthesized transcripts were visualized by Western blot using an antibody targeting puromycin. Actin was used a loading control. Densitometry from n = 4 blots was used to generate a graph representing puromycin labeling of newly synthesized proteins. Error bars represent mean ± SD. *P < 0.1 and **P = 0.05 by one-way ANOVA with Dunnett’s multiple comparisons test. Source data are available for this figure: SourceData FS4.