Relationship between in vivo clone size and in vitro proliferation. The in vivo frequency of each of the indicated clones of infected CD4⁺ T cells compared with its in vitro expansion starting from a single cell. Mean in vitro expansion is plotted for clones encountered in multiple microculture wells (see Table S5). Each data point represents an infected cell clone labeled with its proviral integration site. Symbol shape denotes the detection of HIV-1 virions in vitro. Empty symbols represent clones not detected in vivo and are plotted at 50% of the LOD, which was unique to each clone and proportional to the number of cell equivalents screened (see Table S5 and Materials and methods). Note that outlier LOC100996717 was excluded from the statistical analysis. US indicates the integration site was upstream of the indicated gene by the indicated distance.