Figure 2. IPDA analysis of infected cell... | Rockefeller University Press

Figure 2.

$IPDA analysis of infected cell proliferation. (A) Map of four representative assay plates from two study participants (left, donor 422; right, donor 017) in which CD4+ T cells were distributed into 96-well plates such that each well received on average one infected cell. Colors indicate types of proviruses detected in each well by IPDA analysis, as shown in the key. (B) Map of four representative plates from two study participants (left, donor 383; right, donor 021) in which infected cells were further diluted to 0.35 infected cells/well. Colors indicate types of proviruses detected in each well by IPDA analysis. (C) Pie chart showing the fraction of microculture wells with each provirus type as determined by IPDA for six participants analyzed with plating scheme 1 (n = 1,893 replicate wells). (D) Pie chart showing the fraction of microculture wells with each provirus type as determined by IPDA for four participants analyzed with plating scheme 2 (n = 2,590 replicate wells). (E) Pie charts for individual study participants for plating scheme 1. Numbers indicate participant IDs. (F) Pie charts for individual study participants for plating scheme 2.$

IPDA analysis of infected cell proliferation. (A) Map of four representative assay plates from two study participants (left, donor 422; right, donor 017) in which CD4⁺ T cells were distributed into 96-well plates such that each well received on average one infected cell. Colors indicate types of proviruses detected in each well by IPDA analysis, as shown in the key. (B) Map of four representative plates from two study participants (left, donor 383; right, donor 021) in which infected cells were further diluted to 0.35 infected cells/well. Colors indicate types of proviruses detected in each well by IPDA analysis. (C) Pie chart showing the fraction of microculture wells with each provirus type as determined by IPDA for six participants analyzed with plating scheme 1 (n = 1,893 replicate wells). (D) Pie chart showing the fraction of microculture wells with each provirus type as determined by IPDA for four participants analyzed with plating scheme 2 (n = 2,590 replicate wells). (E) Pie charts for individual study participants for plating scheme 1. Numbers indicate participant IDs. (F) Pie charts for individual study participants for plating scheme 2.