Figure 1.
Currents recording from oocytes expressing KV7.2 channels WT and mutant R198H. (A and B) WT currents were activated with pulses ranging between −120 and +60 mV from a H.P. of −90 mV. (C and D) Equivalent recordings from oocytes expressing the R198H mutant with pulses ranging between −130 and +40 mV. A 5-s prepulse to −120 mV to these recordings as the voltage dependence of the mutant channel was shifted to more negative potentials with respect to WT. To evaluate the effect of external pHEXT, we recorded currents at external pH (pHEXT) ranging between 6.0 (A and C) and 8.0 (B and D). Remarkably, the rate of deactivation of R198H unambiguously decreased at pH 8.0 with respect to pH 6.0, while WT showed more modest changes.

Currents recording from oocytes expressing K V 7.2 channels WT and mutant R198H. (A and B) WT currents were activated with pulses ranging between −120 and +60 mV from a H.P. of −90 mV. (C and D) Equivalent recordings from oocytes expressing the R198H mutant with pulses ranging between −130 and +40 mV. A 5-s prepulse to −120 mV to these recordings as the voltage dependence of the mutant channel was shifted to more negative potentials with respect to WT. To evaluate the effect of external pHEXT, we recorded currents at external pH (pHEXT) ranging between 6.0 (A and C) and 8.0 (B and D). Remarkably, the rate of deactivation of R198H unambiguously decreased at pH 8.0 with respect to pH 6.0, while WT showed more modest changes.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal