Human CD8 T cells can promote tumor growth in vitro. (A) Expression of TOX in TILs. (B) Wound healing assay with SN derived from CEA-CAR-transgenic CD8 T cells versus Ctrl-CAR-transgenic CD8 T cells or no T cells; individual donors are shown. (C) Spheroid assay using cell-free SN from influenza-specific CD8 T cells and varying amounts of pulsed peptides on MRC-5 and HaCaT cells seeded in a 30:70 ratio (left) or tumor-TIL coculture (right); experimental repeat of Fig. 6, B and C.