CD8 T cells instruct other cell types to produce regenerative molecules. (A) Left, PCA of RNA-seq of fibroblast (MRC-5) cells stimulated o/n with SN of MRC-5 cells pulsed with varying concentrations of influenza peptide and cultured with influenza-specific T cells o/n (n = 3); Right, MA-plots with number of DEGs (Padj < 0.05) based on Deseq2 in red, several genes labeled. Gene expression data and statistical analysis in Table S1. (B) MA-plot of primary human fat fibroblasts stimulated with SN generated from autologous CD8 T cells isolated from fat tissue and stimulated o/n with IL-2 and beads or empty medium ctrl (n = 4). DEGs in red. Gene expression data and statistical analysis in Table S2. (C) Left, PCA of RNA-seq of epithelial cells (HaCaT) stimulated o/n with SN of MRC-5 cells pulsed with varying concentrations of influenza peptide and cultured with influenza-specific T cells o/n; Right, MA-plots with number of DEGs (Padj < 0.05) based on Deseq2 in red (n = 4), several genes labeled. Gene expression data and statistical analysis are in Table S3. (D) GSEA of MRC-5 fibroblasts (top) or HaCaT epithelial cells (bottom) stimulated o/n with SN of MRC-5 cells pulsed with 100 ng/ml of influenza peptide and cultured with influenza-specific T cells o/n. (E) Gene expression of IDO1 and IFIT3 in RNA-seq of epithelial cells (HaCaT) stimulated o/n with SN of MRC-5 cells pulsed with varying concentrations of influenza peptide and cultured with influenza-specific T cells o/n. Statistical analysis via Deseq2 (n = 3–4). (F) Relative expression of IDO1 and IFIT3 determined by quantitative PCR in epithelial cells (HaCaT) stimulated o/n in the presence of anti-IFN-γ or IgG control with SN of MRC-5 cells pulsed with 0 or 100 ng/ml of influenza peptide and cultured with influenza-specific T cells o/n. Statistical analysis via one-way ANOVA (n = 3–4).
Figure 2.

CD8 T cells instruct other cell types to produce regenerative molecules. (A) Left, PCA of RNA-seq of fibroblast (MRC-5) cells stimulated o/n with SN of MRC-5 cells pulsed with varying concentrations of influenza peptide and cultured with influenza-specific T cells o/n (n = 3); Right, MA-plots with number of DEGs (Padj < 0.05) based on Deseq2 in red, several genes labeled. Gene expression data and statistical analysis in Table S1. (B) MA-plot of primary human fat fibroblasts stimulated with SN generated from autologous CD8 T cells isolated from fat tissue and stimulated o/n with IL-2 and beads or empty medium ctrl (n = 4). DEGs in red. Gene expression data and statistical analysis in Table S2. (C) Left, PCA of RNA-seq of epithelial cells (HaCaT) stimulated o/n with SN of MRC-5 cells pulsed with varying concentrations of influenza peptide and cultured with influenza-specific T cells o/n; Right, MA-plots with number of DEGs (Padj < 0.05) based on Deseq2 in red (n = 4), several genes labeled. Gene expression data and statistical analysis are in Table S3. (D) GSEA of MRC-5 fibroblasts (top) or HaCaT epithelial cells (bottom) stimulated o/n with SN of MRC-5 cells pulsed with 100 ng/ml of influenza peptide and cultured with influenza-specific T cells o/n. (E) Gene expression of IDO1 and IFIT3 in RNA-seq of epithelial cells (HaCaT) stimulated o/n with SN of MRC-5 cells pulsed with varying concentrations of influenza peptide and cultured with influenza-specific T cells o/n. Statistical analysis via Deseq2 (n = 3–4). (F) Relative expression of IDO1 and IFIT3 determined by quantitative PCR in epithelial cells (HaCaT) stimulated o/n in the presence of anti-IFN-γ or IgG control with SN of MRC-5 cells pulsed with 0 or 100 ng/ml of influenza peptide and cultured with influenza-specific T cells o/n. Statistical analysis via one-way ANOVA (n = 3–4).

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