γ-oscillations in acute slices from Nedd4 brain-specific knockout mice. (A–D) Reduced power of γ-oscillations in Nedd4-1^f/f;Nedd4-2^f/f;EMX-Cre (N4-1/2 bDKO) mice. Representative recordings in CA3 hippocampal regions of acute brain slices from Nedd4-1^f/f;Nedd4-2^f/f (N4-1/2 CTL) (A) and N4-1/2 bDKO (B) mice before (baseline) and during (Kainate) induction of γ-oscillations with 100 nM kainate. Average powers (C) and frequencies (D) of γ-oscillations in N4-1/2 CTL (black dots) and N4-1/2 bDKO (green dots). (E–H) Reduced power of γ-oscillations in Nedd4-2^f/f;EMX-Cre (N4-2 bKO) mice. Representative recordings in the CA3 region of hippocampus slices from Nedd4-2^f/f (N4-2 CTL) (E) and N4-2 bKO (F) mice before (Baseline) and during (Kainate) the application of 100 nM kainate. Average powers (G) and frequencies (H) of γ-oscillations in N4-2 CTL (black dots) and N4-2 bKO (green dots). Different tones and shapes of dots in dot plots in C, D, G, and H represent data from different mice. Numbers of recorded slices (n) and the animal number (N); (C and D), n = 16 and N = 3 for N4-1/2 CTL, n = 21 and N = 3 for N4-1/2 bDKO; (G and H), n = 29 and N = 6 for N4-2 CTL, n = 25 and N = 5 for N4-2 bKO. Results are shown as mean ± SEM. **, 0.001 < P < 0.01; *, 0.01 < P < 0.05; ns, 0.05 < P (two-tailed nested t test). Data distribution was assumed to be normal but this was not formally tested. See also Fig. S1 and Table S2.