Live imaging of lysosomes and mitochondria in mouse embryonic lung epithelial cells in explant culture. (A) Native fluorescence from the three fusion proteins in ROSAKaleidoscope activated in lung epithelial progenitors (Sox9CreER; Tam, 500 μg tamoxifen) at time 40 min (t40) in explant culture. Asterisk, artifact fluorescence. The boxed region is shown over time (t in minutes). Colored arrowheads track the same objects, consistent with lysosome fusion and fission as well as mitochondrial restructuring. Scale: 10 μm. See also Videos 3, 4, 5, and 6. (B) Preservation of native fluorescence over 4 h of live imaging. Lysosomal signal is more robust than mitochondrial and microtubule signals. Scale: 10 μm.
Figure 5.

Live imaging of lysosomes and mitochondria in mouse embryonic lung epithelial cells in explant culture. (A) Native fluorescence from the three fusion proteins in ROSAKaleidoscope activated in lung epithelial progenitors (Sox9CreER; Tam, 500 μg tamoxifen) at time 40 min (t40) in explant culture. Asterisk, artifact fluorescence. The boxed region is shown over time (t in minutes). Colored arrowheads track the same objects, consistent with lysosome fusion and fission as well as mitochondrial restructuring. Scale: 10 μm. See also Videos 3, 4, 5, and 6. (B) Preservation of native fluorescence over 4 h of live imaging. Lysosomal signal is more robust than mitochondrial and microtubule signals. Scale: 10 μm.

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