Cell type–specific sizes and distributions of lysosomes and mitochondria in the lung. (A) Genetic labeling and Imaris 3D rendering of lysosomes, mitochondria, and microtubules in individual AT2 (Sftpc^CreER; 100 μg tamoxifen at P1 and harvested at P7), AT1 (Rtkn2^CreER; 50 μg tamoxifen at P1 and harvested at P11), and Cap2 (Car4^CreER; 100 μg tamoxifen at P3 and harvested at P12) cells. n, nucleus of labeled cells based on exclusion of EGFP-α-Tubulin; dash, AT1 cell boundary; caret, Cap2 cell edge with few lysosomes and mitochondria. Scale: 10 μm. See also Videos 1 and 2. (B) Population and single-cell level measurements with means and standard deviations of lysosomes and mitochondria in genetically labeled AT2, AT1, and Cap2 cells. Individual lysosomal or mitochondrial clusters (defined by Imaris segmentation) of AT2 cells are larger than those of AT1 and Cap2 cells (asterisk, P < 0.0001). AT1 cells contain more lysosomal and mitochondrial clusters per cell than AT2 and Cap2 cells (asterisk, P < 0.001). Lysosomes and mitochondria are closer to the nucleus in AT2 cells than in AT1 and Cap2 cells, attributable to cell size differences (asterisk, P < 0.0001). GFP volume ratio is calculated as the percentage of cell volume, approximated by GFP, occupied by lysosomes or mitochondria, and differs among cell types. Statistics is based on ordinary ANOVA with Tukey test.