ROSA ^Kaleidoscope captures stereotypic mitotic microtubule structures. (A) Primary cell culture from dissociated P7 CMV-Cre; ROSA^{Kaleidoscope/+} lungs with a mitotic spindle labeled with the EGFP-α-Tubulin fusion protein and colocalized with β-Tubulin immunostaining, in contrast to their distribution in the adjacent interphase cell. Scale: 5 μm. (B) Genetically labeled AT2 cells showing the distributions of EGFP-α-Tubulin fusion protein through the cell cycle stages. Arrowhead, centrosomal microtubules. Dividing daughter cells are outlined with dashes. Mitochondria fill both daughter cells, whereas lysosomes could be asymmetric. Tam, 100 μg tamoxifen. Scale: 5 μm.