CCDC15 is stably associated with centrosomes throughout the cell cycle. (A) Localization of endogenous CCDC15 to the centrioles in different cell cycle stages. RPE1 cells were stained with antibodies against the centriole marker Centrin-2 and CCDC15. DNA was visualized with DAPI. Scale bar, 5 μm; insets, 1 μm. (B) Spatiotemporal dynamics of CCDC15 during cell cycle. RPE1 cells stably expressing mNG-CCDC15 during mNG-CCDC15 were imaged every 2 min. Scale bar, 1 μm; insets, 0.25 μm. (C) FRAP analysis of CCDC15 dynamics at centrioles. RPE1 cells stably expressing mNG-CCDC15 were grown in a glass-bottom dish; centrioles indicated with yellow circles (3 μm²) were photobleached and then assessed at the indicated times after photobleaching. Scale bar, 500 nm. (D) Percentage of recovery graph of C. Individual FRAP experiments from two biological replicates were fitted into one-phase association curves. n = 11 cells for one centriole curve and n = 15 cells for two centrioles curve. (E) Half-time analyses were calculated using recovery data from D. Error bars, SD. (F) Percentage of mobile and immobile pools of CCDC15 at centrioles were calculated from D. Error bars, SD.