The effect of puromycin treatment on cell abscission. (A and B) HeLa cells were fixed and subjected to immunostaining using anti-acetylated-tubulin. A represents a cell in telophase. B represents a cell that had just undergone abscission (see arrow). The lines used for the line intensity graphs are marked in yellow, and the white dashed square represents the region of the image used for the inset. Scale bars: 10 μm. (C and D) Line intensity graphs representing the intensity of acetylated-tubulin in telophase and abscission cells. The abscission site is marked by an arrow (D). (E and F) Telophase HeLa cells stably expressing GFP-MKLP1 were analyzed by time-lapse microscopy. Cells were imaged for 120 min with 15 min time-lapse. Scale bars: 10 μm.