MBsomes are enriched in mRNAs encoding proteins that regulate translation and cell cycle. (A) The Gene Ontology (GO) pathway analysis tool was used to determine the pathways associated with the enriched MB-associated mRNAs. (B) MB enrichment of mRNAs encoding known abscission regulators. Data shown present means and SD derived from three separate RNAseq analyses. Dashed line marks the mRNA levels present in the whole cell transcriptome. (C) Whole-cell or MBsome-associated mRNAs were isolated from GFP-MKLP1-expressing HeLa cell line. The cDNA was subject to RT-qPCR using TSG101, CHMP4B, CHMP2A, CHMP3, CHMP6, or GAPDH (control) specific primers and represented as normalized values. The means and SD were calculated from three independent experimental repeats. Statistical analysis is represented with a P value. (D) Schematic representing key regulators of abscission and how they relate to each other in the intercellular bridge.