Map of TZ and TF components of epidermal motile cilia. (A) Airyscan super-resolution Z-stack images of MCCs expressing the transition zone proteins (i) mTagBFP2-Centrin and mCherry-CEP290, (ii) mEmerald-B9D1 and mCherry-CEP290, (iii) mTagBFP2-Centrin, mEmerald-B9D1 and mCherry-CEP290, (iv) mEmerald-NPHP1 and mCherry-CEP290, (v) mEmerald-MKS1 and mCherry-CEP290, (vi) mEmerald-MKS1 and mCherry-TMEM216, and (vii) mCherry-TMEM216 and stained for CEP164. Scale bars, 5 μm. (B) Representative Z-stack images of MCCs expressing the transition fiber proteins (i) GFP-Chibby and mCherry-CEP290, (ii) GFP-Chibby stained for CEP164, (iii) GFP-CEP123 and mCherry-CEP290, (iv) GFP-CEP123 stained for CEP164, (v) GFP-IFT52 stained for CEP164, (vi) GFP-CEP83 stained for CEP164, and (vii) GFP-CEP83 and mCherry-CEP290. Scale bars, 5 μm. (C) Airyscan super-resolution Z-stack images of MCCs expressing (i) GFP-JNK1 and mCherry-TMEM216, (ii) mEmerald-MKS1 stained for pJNK, (iii) GFP-JNK1 and mCherry-CEP290, and (iv) mEmerald-B9D1 stained for pJNK. Scale bars, 5 μm. (D) Multiciliated cell expressing mScarlet JNK (acceptor) and GFP-Chibby. GFP intensity remains unchanged suggesting that JNK is not associated with Chibby. Data represent mean ± SEM (n = 6 MCCs from three embryos). Scale bars, 5 μm. (E) Western blot showing that immunoprecipitated GFP-JNK from uninjected and Multicilin overexpressing skin lysates interacts with CEP164. Source data are available for this figure: SourceData FS2.