TIMP1 inhibits ADAM10 proteolytic activity and Notch signaling in sham and CL&P LSK cells. Related to Fig. 6. (A) Schematic outline of the colorimetric assay. Proteolytic activity of ADAM10 in the presence or absence of inhibitor GI254023X or TIMP1 was measured by a colorimetric assay. Symbols depict data from three independent experiments with bars showing the mean value ± SD. **** P < 0.0001; *** P < 0.001 (unpaired two-tailed Student’s t test). (B) Top panel shows proteolytic activation of Notch signaling. Bottom violin plots show the equal expression of Notch receptors, Notch sheddases, and Notch co-activators in sham and CL&P LSK cells. (C) GSEA of the Notch signaling pathway gene signature in sham versus CL&P LSK cells.