The hematopoietic microenvironment of mice surviving sepsis enhances myeloid output. (A) BM cells from B6 mice at 12 wk after sham or CL&P surgery were transferred into irradiated B6.SJL mice, respectively. 6 wk later, FACS analysis was performed to determine the total number of myeloid cells (Gr and Mono) and lymphocytes (B and T cells) in the spleen. Each symbol represents one mouse, with eight mice engrafted with sham donor cells and six mice with CL&P donor cells (data combined from two independent experiments). Bars show the mean value ± SD. ns, P > 0.05 (unpaired two-tailed Student’s t test). (B) BM cells from B6.SJL mice were transferred into irradiated B6 mice at 12 wk after sham surgery (n = 9) or CL&P surgery (n = 8). 6 wk later, FACS analysis was performed to determine the total numbers of myeloid cells (Gr and Mono) and lymphocytes (B and T cells) in the spleen. Symbols represent each mouse and data were collected from two independent experiments. Bars represent the mean value ± SD. * P < 0.05; ns, P > 0.05 (unpaired two-tailed Student’s t test). (C) MPP3 or MPP4 cells from B6.SJL mice were transferred into sublethally irradiated naïve B6 mice. 14 days later, FACS analysis was performed to determine the frequencies of donor derived CD45.1+ CD11b+ myeloid cells and CD45.1+ B cells in the peripheral blood. Data were collected from five recipient mice transplanted with MPP3 cells and six mice transplanted with MPP4 cells. Bars represent the mean value ± SD. **** P < 0.0001 (unpaired two-tailed Student’s t test). (D) MPP4 cells from B6.SJL mice were transferred into sublethally irradiated sham (n = 6) or CL&P (n = 4) mice at 12 wk after surgery. 14 days later, FACS analysis was performed to determine the frequencies of donor-derived CD45.1+ CD11b+ myeloid cells and CD45.1+ B cells in the peripheral blood. Bars represent the mean value ± SD. * P < 0.05 (unpaired two-tailed Student’s t test).
Figure 4.

The hematopoietic microenvironment of mice surviving sepsis enhances myeloid output. (A) BM cells from B6 mice at 12 wk after sham or CL&P surgery were transferred into irradiated B6.SJL mice, respectively. 6 wk later, FACS analysis was performed to determine the total number of myeloid cells (Gr and Mono) and lymphocytes (B and T cells) in the spleen. Each symbol represents one mouse, with eight mice engrafted with sham donor cells and six mice with CL&P donor cells (data combined from two independent experiments). Bars show the mean value ± SD. ns, P > 0.05 (unpaired two-tailed Student’s t test). (B) BM cells from B6.SJL mice were transferred into irradiated B6 mice at 12 wk after sham surgery (n = 9) or CL&P surgery (n = 8). 6 wk later, FACS analysis was performed to determine the total numbers of myeloid cells (Gr and Mono) and lymphocytes (B and T cells) in the spleen. Symbols represent each mouse and data were collected from two independent experiments. Bars represent the mean value ± SD. * P < 0.05; ns, P > 0.05 (unpaired two-tailed Student’s t test). (C) MPP3 or MPP4 cells from B6.SJL mice were transferred into sublethally irradiated naïve B6 mice. 14 days later, FACS analysis was performed to determine the frequencies of donor derived CD45.1+ CD11b+ myeloid cells and CD45.1+ B cells in the peripheral blood. Data were collected from five recipient mice transplanted with MPP3 cells and six mice transplanted with MPP4 cells. Bars represent the mean value ± SD. **** P < 0.0001 (unpaired two-tailed Student’s t test). (D) MPP4 cells from B6.SJL mice were transferred into sublethally irradiated sham (n = 6) or CL&P (n = 4) mice at 12 wk after surgery. 14 days later, FACS analysis was performed to determine the frequencies of donor-derived CD45.1+ CD11b+ myeloid cells and CD45.1+ B cells in the peripheral blood. Bars represent the mean value ± SD. * P < 0.05 (unpaired two-tailed Student’s t test).

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