HSPCs in mice surviving sepsis are active and acquire alternative myeloid differentiation potential. (A) A waterfall plot shows biological processes and pathways enriched in sham LSK cells (red) and in CL&P LSK cells (blue). (B) Top FACS plots show cell cycle analysis of sham and CL&P LSK cells. Bottom panel shows statistical results with each dot representing data from an individual mouse (sham = 4, CL&P = 4; data from two independent experiments). Bars representing the mean value ± SD. * P < 0.05; ns, P > 0.05 (unpaired two-tailed Student’s t test). (C and D) PCA for sham and CL&P LSK cells colored by the cell state (C), or by trajectory leaf nodes (D). Graphs are shown with logarithmic axes. Red arrows point to cells aberrantly expanded in the CL&P sample. (E) Heatmap of expression of the core signature genes of leaf node 2 (row) in sham and CL&P LSK cells (column) ordered by pseudotime. Annotations by the leaf nodes (1) or by the cell states (2) are displayed at the top. Boxes in the heatmap highlight transcripts upregulated in CL&P LSK cells. (F) Projection of Ms4a3 and Satb1 expression on the PCA plots. (G) Scatterplots display co-expression of the lymphoid commitment gene Satb1 and the myeloid commitment gene Ms4a3 in LSK cells.