Increased myeloid output in the BM of mice 12 wk after CL&P surgery. (A) The top left graph shows the numbers of BM cells from two pairs of femurs and tibias of each mouse. The FACS plots and the middle panel of histograms show percentages of monocytes (Mono), granulocytes (Gr), and B cells. The right histogram panels show the statistics of the total numbers of monocytes, granulocytes, and B cells in two pairs of femurs and tibias per mouse. Data of sham (n = 8) and CL&P mice (n = 12) were generated from three independent experiments. Each dot represents an individual mouse and bars depict the average cell counts ± SD. ** P < 0.01; *** P < 0.001 (unpaired two-tailed Student’s t test). (B) The absolute numbers of LSK, LT-HSCs, ST-HSCs, MPP2, MPP3, and MPP4 cells in sham and CL&P mice. Symbols show data of individual mice; bars show the mean value ± SD. * P < 0.05; ** P < 0.01; ns, P > 0.05. (C) Percentages of LSK cells are shown in the left panel. Proportions of HSC and MPP subsets within the LSK population are shown in the right histograms with stacked bars. Data are expressed as mean value ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, P > 0.05. (D) The ratio of MPP3 to MPP4 cells. Symbols show data of individual mice; bars show the mean value ± SD. *** P < 0.001. (B–D) Data of six sham and nine CL&P mice were pooled from two independent experiments. P values by unpaired two-tailed Student’s t test are indicated.