Changes in the hematopoietic compartments in mice at different time points after sham or CL&P surgery. Related to Fig. 1. (A) Kaplan–Meier survival curves of sham and CL&P mice. Data were from three independent experiments. (B) The total number of splenic B cells in mice at the indicated time points after surgery (10 days: n = 3/group; 2 wk: n = 6/group; 10–12 wk: n = 8/group). Symbols represent data of individual mice collected from two experiments. Bars depict the mean value ± SD. ** P < 0.01; ns, P > 0.05 (unpaired two-tailed Student’s t test). (C) The total number of splenic T cells in mice at different time points after surgery as indicated in B. Symbols represent data from individual mice; bars depict the mean value ± SD. ** P < 0.01; *** P < 0.001; ns, P > 0.05 (unpaired two-tailed Student’s t test). (D) The left panel shows representative FACS plots depicting percentages of CD11b⁺Ly6C⁺ monocytes (Mono) or Ly6G⁺ granulocytes (Gr) in the spleen of mice at week 12 after surgery. The right panel shows the statistics of the total number of monocytes and granulocytes. Symbols show the total numbers of monocytes or granulocytes in individual mice (n = 4–8); bars depict the mean value ± SD. ** P < 0.01; *** P < 0.001; **** P < 0.0001 (unpaired two-tailed Student’s t test). (E) Symbols represent the total number of circulating monocytes or granulocytes in the blood of individual mice 2 wk after surgery (sham = 6, CL&P = 13) or 29 wk after surgery (sham = 3, CL&P = 7). Bars depict the mean value ± SD. * P < 0.05; **** P < 0.0001 (unpaired two-tailed Student’s t test). (F) Gating strategy for LSK, LT-HSC, ST-HSC, MPP2, MPP3, and MPP4 cells. Representative plots are shown for sham and CL&P mice. Percentages of HSPC subsets were indicated.