Distinct synaptic phenotypes of the R359C and the Q801P mice. (A and B) Representative recordings of the evoked EPSCs and plots showing the mean amplitudes (pA) of the EPSCs versus the stimulus intensities (mV) for CA1 pyramidal neurons in the slices from the R359C and Q801P mutant and their respective WT control littermates. Data are presented as mean ± SEM (n = 17–29 recordings per eight mice per group; ***P < 0.001; unpaired t test). (C and D) Representative traces and plots showing the frequency and the mean amplitude of the miniature and the spontaneous EPSCs recorded from CA1 pyramidal neurons from the R359C or Q801P mutant mice and their respective WT littermates. Data are presented as mean ± SEM (n = 18–25 recordings per eight mice per group; n.s., not significant; *P < 0.05; ****P < 0.0001; unpaired t test). (E and F) Plots showing the time course (left) and the summarized values 30 min after each tetanus (right) of the mean ± SEM (n = 15–24 recordings per five to eight mice per group; **P < 0.01; unpaired t test) of the 5–95% fEPSP slope, normalized to the baseline (defined as 1.0) immediately preceding first tetanus (↑) in the hippocampal slices from the R359C and Q801P mutant mice and their respective WT control littermates. Sample traces (inserts) show the baseline and sweeps at 15 min following tetani. Scale bars: 1 mV, 15 ms. (G) A plot showing the time course of the mean ± SEM (n = 15–25 recordings per five to eight mice per group) of the 5–95% fEPSP slope, normalized to the baseline (defined as 1.0) immediately preceding the low-frequency stimulation (LFS) in the hippocampal slices from the R359C and Q801P mutant mice and their respective WT littermates. Sample traces before and 30 min after LFS are shown at the top of the plot.