Gradual acquisition of the SILP ILC2 phenotype by transferred kidney ILC2s. (A) Unsupervised UMAP subclustering of scRNAseq data of ex-kidney ILC2s isolated from the SILP of recipient BALB/c Rag2−/−Il2rg−/− mice 2 wk after transfer (see Fig. 4 A for experimental setup). (B) Color mapping of kidney and SILP program expression on the UMAP subclustering of adapting ex-kidney ILC2s at 2 wk after transfer. Lowest expression is indicated by blue and highest expression by yellow. (C) Dynamic information of cell states in the transition process generated using RNA velocity analysis of adapting ex-kidney ILC2s at 2 wk after transfer. (D and E) RNA velocity-based pseudotime analyses of the SILP and kidney programs (D) and selected surface markers (E) in adapting ex-kidney ILC2s at 2 wk after transfer.