Figure S4.

Cluster-defining genes and pathway analysis of scRNAseq data. (A) Heat map depicting top 10 cluster-defining genes for each cluster obtained from scRNAseq analysis of donor kidney and SILP ILC2s as well as recipient SILP ILC2s (see Fig. 4 A for experimental setup). Genes associated with RA and Notch signaling are marked with an arrow and labeled in red. (B) Gene set enrichment analysis (GSEA) for HALLMARK pathways of the Mouse Molecular Signatures Database using the genes significantly upregulated in the indicated comparisons between ILC2 populations (see Fig. 4 A for experimental setup). Specific pathways identified by further filtering (see Fig. 4, G and H) are indicated by blue boxes and marked in bold.

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