Figure S3.
Sorting purity and scRNAseq analysis of ILC2s. (A) Representative sorting purity of ILC2s (sorted as Lin−CD127+IL-13fm+) isolated from the kidney and SILP of IL-33–treated BALB/c IL-13fm mice (donors) for scRNAseq. (B) Representative sorting purity of ILC2s (sorted as Lin−CD127+IL-13fm+) isolated from the SILP (2 and 8 wk after transfer) of BALB/c Rag2−/−Il2rg−/− mice that received adoptive i.v. transfer of kidney ILC2s. (C) Unsupervised UMAP clustering of kidney ILC2s from donor mice. (D) Heat map of top 15 cluster-defining genes obtained from C. (E) Feature plots depicting expression of key markers on the UMAP of the kidney ILC2 dataset from C. (F) Overlay of Tcf7 and Il18r1 genes known to be co-expressed in ILC precursors.

Sorting purity and scRNAseq analysis of ILC2s. (A) Representative sorting purity of ILC2s (sorted as Lin−CD127+IL-13fm+) isolated from the kidney and SILP of IL-33–treated BALB/c IL-13fm mice (donors) for scRNAseq. (B) Representative sorting purity of ILC2s (sorted as Lin−CD127+IL-13fm+) isolated from the SILP (2 and 8 wk after transfer) of BALB/c Rag2−/−Il2rg−/− mice that received adoptive i.v. transfer of kidney ILC2s. (C) Unsupervised UMAP clustering of kidney ILC2s from donor mice. (D) Heat map of top 15 cluster-defining genes obtained from C. (E) Feature plots depicting expression of key markers on the UMAP of the kidney ILC2 dataset from C. (F) Overlay of Tcf7 and Il18r1 genes known to be co-expressed in ILC precursors.

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