Effector ILC2s can adapt to new tissue microenvironment. (A) Flow cytometric analysis of IL-5^tdtomato+GATA3⁺ ILCs isolated from kidney and SILP of IL-33–treated Red5 mice (i.p. injection on four consecutive days). Numbers indicate the percentage of cells in each gate. (B) Heat map of MFI of different surface markers on IL-5^tdtomato+GATA3⁺ ILCs isolated from kidney and SILP normalized to surface marker expression of kidney ILC2s. (C) Representative sorting purity of transferred ILC2s (sorted as CD45⁺Lin⁻CD127⁺IL-33R⁺KLRG1⁺IL-5^tdtomato+) originating from the kidney of IL-33–treated Red5 mice. Sorted kidney ILC2s were transferred to C57BL/6 Rag2^−/−Il2rg^−/−. (D–F) Representative flow cytometric plots (D), frequencies (E), and absolute numbers (F) of IL-5^tdtomato+GATA3⁺ ILC2s obtained from the kidney and SILP (n = 4–7 per organ) of C57BL/6 Rag2^−/−Il2rg^−/− mice at different time points after transfer. Numbers in D indicate the percentage of events in each gate. Symbols represent individual data points, and bars indicate mean ± SEM. (G and H) Representative histogram overlays (G) and heat map of MFI (H) of different surface markers of IL-5^tdtomato+GATA3⁺ ILCs originating from the kidney of Red5 mice analyzed 1–6 wk after transfer in the kidney and SILP of C57BL/6 Rag2^−/−Il2rg^−/− mice and normalized to surface marker expression on kidney ILC2s. (I) Immunohistochemical staining of tdTomato-positive ILC2s (deep red) in the kidney and SILP tissue of IL-33–treated Red5 mice and in C57BL/6 Rag2^−/−Il2rg^−/− mice 3 wk after kidney IL-5^tdtomato+ ILC2 transfer. Scale bars, 50 µm. Data in D–H represent five independent experiments.