Figure 7.
CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥ 400; E n = 3 ROI ≥ 2,500.

CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥ 400; E n = 3 ROI ≥ 2,500.

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