Purification of TRIC-B proteins by density gradient ultracentrifugation. (A) Schematic representation of the proteoliposomes containing TRIC-B proteins by wheat-germ translation system. (B) SDS-PAGE analysis of proteoliposomes. After the synthesis reaction, the reaction mixture was subjected to a density gradient and fractions were collected from the top of the tube. Synthesized proteins in each fraction were applied to SDS-PAGE and stained CBB (upper panel) or transferred PVDF and probed using TRIC-B antibody (lower panel). Sk. m., mouse skeletal muscle protein.