Example of single-channel recordings of TRIC-B expressed in wheat germ. (A) Liposomes containing TRIC-B expressed in the wheat germ system were fused into bilayers under KCl gradient conditions (740 mM KCl cis; 210 mM KCl trans), 2 µM free Ca²⁺. The recording illustrates the typical multiple-channel fluctuations observed in the KCl gradient and at holding potentials of +40 and −40 mV. The dashed line represents the zero current level. The large current observed at −40 mV indicates that TRIC-B is predominantly permeable to Cl⁻ ions. (B) Example of current fluctuations of TRIC-B from wheat germ during the application of a voltage ramp under gradient (pink trace) conditions. E_rev is indicated by the arrow. (C) The mean E_rev values for each bilayer are shown for RyR2+TRIC-B (red triangles; n = 13; data from Fig. 9), RyR2+TRIC-B without outliers (orange triangles; n = 11) and TRIC-B expressed in wheat germ (pink squares; n = 8). One-way ANOVA shows no significant difference with P value = 0.3678.