Effect of co-expressing TRIC-A or TRIC-B on background currents. (A) The bar chart illustrates the percentage of the bilayer in which no background current was detected after the incorporation of vesicles from HEK293 cells expressing RyR2-only, RyR2+TRIC-A, and RyR2+TRIC-B. Significant differences were detected across the three groups using a χ-square test with a P value of 0.000065. The percentage of bilayers with no background current is indicated in each bar (total number of bilayers: n_RyR2 = 21, n_RyR2+TRIC-A = 24, n_RyR2+TRIC-B = 26). (B) Mean current amplitudes in KCl gradient conditions after vesicles incorporation are compared for HEK293 cells expressing RyR2-only, RyR2+TRIC-A, and RyR2+TRIC-B. One-way ANOVA: P = 0.000065. Post-hoc multiple comparison with Tukey’s correction: significant differences between RyR2-only and RyR2+TRIC-A vesicle preparations are indicated by *** for P = 0.0003 and between the RyR2+TRIC-A and RyR2+TRIC-B indicated by *** with P = 0.0005 (n_RyR2 = 21, n_RyR2+TRIC-A = 24, n_RyR2+TRIC-B = 26). Symbols indicate values from individual experiments and bars indicate mean ± SEM.