The effects of co-expressing TRIC-A or TRIC-B with RyR2 on the frequency of subconductance events. Bar chart showing the frequency of subconductance open events >2 ms in duration at 10 µM cytosolic free [Ca²⁺] at −30 mV, in the same solutions as in Fig. 2, for RyR2 derived from HEK293 cells expressing RyR2-only (black), RyR2+TRIC-A (blue), and RyR2+TRIC-B (red). The Kruskal–Wallis test for one independent variable, type of cell, P = 0.0271. Post-hoc multiple comparison with Dunn’s correction: significant difference between the event frequency of RyR2 derived from HEK293 cells expressing RyR2-only or cells expressing RyR2+TRIC-A is indicated by * for P = 0.0287 (n_RyR2 = 6, n_RyR2+TRIC-A = 6, n_RyR2+TRIC-B = 8). Symbols indicate values from individual experiments and bars show mean ± SEM.