Expression of TRIC channels and their interaction with RyR2. (A) Western blots of the microsomal membrane vesicles isolated from RyR2-expressing HEK293 cells that were either uninfected (left), infected with TRIC-A baculovirus (BV; center), or TRIC-B BV (right). (B) Interaction of TRIC channels with RyR2. SBP-tagged RyR2-expressing HEK293 cells were infected with TRIC-A (left) or TRIC-B (right) BV. The cell lysate was immunoprecipitated with control IgG or anti-SBP antibody. Lysate and the immunoprecipitated product were analyzed by Western blotting. Note that both TRIC-A and TRIC-B were coimmunoprecipitated with RyR2. Source data are available for this figure: SourceData FS1.