Dsg1 and its regulators are associated with intraepidermal spread of melanoma cells in vivo. (A) Paraffin-embedded sections of melanoma lesions were stained for Dsg1, Grhl1, Slug, or Ecad and Mel-A. (B and C) The number of individual melanoma cells spreading away from the basal layer, (B) as well as the total number of melanoma cells in each section (C) were counted and then correlated to the staining intensity of Dsg1, Grhl1, Slug, and Ecad. (Note that the E-cadherin/Mel-A stained tumor sample in the bottom panel was also used in Fig. 1 A for analysis of E-cadherin fluorescence intensity in proximal versus distal keratinocytes.) n > 10. Simple linear regression.