Modulation of melanoma migration is correlated with Dsg1 levels in keratinocytes. Conditioned media from primary keratinocytes derived from four separate donors was used to treat melanoma cells in migration assays. Dsg1 knockdown and rescue were not equal in each clone. Shown is a graph of the average migration increase in melanoma cells upon treatment with shDsg1 conditioned media or shDsg1 + FL conditioned media compared with control, correlated with the keratinocyte Dsg1 protein levels in the clones used for each in the shDsg1 cells (purple) and the shDsg1 + FL cells (blue).