IL-33 and IL-34 also show age- and diet-related differences in the rostral NST. (A) Photomicrographs of an optical, horizontal section in the rostral NST (outlined in white) in a P22 control and E3–E12 mouse showing IL-34⁺ staining (red) in nuclei of NST neurons (blue; left panels) and only with IL-34⁺ staining. (B) Percent of NST neurons containing IL-34⁺ staining in neuronal nuclei in the rostral NST in P22 controls and E3–E12 diet (n = 6, 9, respectively) and in P40 control and E3–E12 diet mice (n = 6, 7, respectively). (C) NST neuron densities in the rostral NST in P22 controls and E3–E12 diet (n = 6, 9, respectively) and in P40 control and E3–E12 diet mice (n = 6, 7, respectively). (D) Photomicrographs of the rostral NST (outlined in white) in a P22 control and E3–E12 mouse showing IL-33⁺ staining (red) in nuclei of NST neurons (blue; left panels) and only with IL-33⁺ staining. For all dietary group comparisons, statistical comparisons were multiple, unpaired t tests using the Holm–Sidak method for multiple comparisons. (C) * denotes P = 0.02 and 0.003 for P22 versus adult for controls and E3–E12 diet mice, respectively. Scale bars in A and D = 100 µm.