Expanded photomicrographs of terminal fields in the NST in control mice treated with PLX5622 or E3–E12 diet mice treated with m-csf. (A) Horizontal sections of labeled terminal fields of the IX, GSP, CT, and the merged image in the far dorsal, dorsal, intermediate, and ventral zones in control mice fed the control diet or the diet with PLX5622. The photomicrographs for the intermediate zone in Fig. S4 A are duplicated from Fig. 4 C. (B) Microglia densities (mean ± SEM) in P15–P40 mice fed the control diet or the diet containing PLX5622 from P18–P40. The numbers/group for controls and E3–E12 diet mice were P15 (7, 7, respectively), P19 (3, 4, respectively), P21 (6, 5, respectively), P23 (4, 3, respectively), P25 (3, 4, respectively), and P40 (7, 4, respectively). (C) Horizontal sections of P25 control mice fed the control diet (left) or the diet containing PLX5622 (right). The white outline denotes the rostral border of the NST. Scale bar = 100 µm. (D) Horizontal sections of labeled terminal fields of the IX, GSP, CT, and the merged image in the far dorsal, dorsal, intermediate, and ventral zones in E3–E12 diet mice injected with saline or with m-csf from P10–P40. Scale bars in A and D = 200 µm. The photomicrographs for the intermediate zone in Fig. S4 D are duplicated from Fig. 4 E.
Figure S4.

Expanded photomicrographs of terminal fields in the NST in control mice treated with PLX5622 or E3–E12 diet mice treated with m-csf. (A) Horizontal sections of labeled terminal fields of the IX, GSP, CT, and the merged image in the far dorsal, dorsal, intermediate, and ventral zones in control mice fed the control diet or the diet with PLX5622. The photomicrographs for the intermediate zone in Fig. S4 A are duplicated from Fig. 4 C. (B) Microglia densities (mean ± SEM) in P15–P40 mice fed the control diet or the diet containing PLX5622 from P18–P40. The numbers/group for controls and E3–E12 diet mice were P15 (7, 7, respectively), P19 (3, 4, respectively), P21 (6, 5, respectively), P23 (4, 3, respectively), P25 (3, 4, respectively), and P40 (7, 4, respectively). (C) Horizontal sections of P25 control mice fed the control diet (left) or the diet containing PLX5622 (right). The white outline denotes the rostral border of the NST. Scale bar = 100 µm. (D) Horizontal sections of labeled terminal fields of the IX, GSP, CT, and the merged image in the far dorsal, dorsal, intermediate, and ventral zones in E3–E12 diet mice injected with saline or with m-csf from P10–P40. Scale bars in A and D = 200 µm. The photomicrographs for the intermediate zone in Fig. S4 D are duplicated from Fig. 4 E.

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