Depletion of UHRF1 damages spindle architecture. (A) DU145 cells were transiently transfected with or without siUHRF1. The mitotic spindles were stained with immunofluorescent anti-α-tubulin antibody (green) and chromosomes were stained with DAPI. Scale bar, 5 μm. (B–D) Spindle pole distance was measured. n = 40 cells (B). The percentage of cells at the metaphases with abnormal spindle geometry was assessed (C) in DU145 cells when transfected with or without siUHRF1. n > 50 cells for each condition. The asters fluorescence intensity of the metaphase spindle was measured. n = 40 cells for each condition. (E) DU145 cells with UHRF1 depletion were transiently transfected with plasmids expressing UHRF1^ΔRING or UHRF1^WT. The mitotic spindles were stained with immunofluorescent α-tubulin antibody and chromosomes were stained with DAPI. Scale bar, 5 μm. (F–H) Spindle pole distance was measured (n = 40 cells; F) and the percentage of cells at the metaphases with abnormal spindle geometry was assessed (n > 50 cells; G). The mean fluorescence intensity of asters was analyzed by ImageJ. n = 40 cells (H). The data for quantification in C, D, G, and H are from n = 3 independent experiments. Results are represented as mean ± SD; error bars represent SD. Dots represent individual cell samples in B and F; bars are median ± quartile. n.s., not significant; ***, P < 0.001. One-way ANOVA test.