In vitro recombinant proteins GST-pull down assay. (A) Knockdown of UHRF1. DU145 cells were transiently transfected with UHRF1 siRNAs and UHRF1 expression was evaluated by Western blotting. (B) GST-pulldown assay of UHRF1 using the indicated GST fusion proteins. EG5 protein was detected. Source data are available for this figure: SourceData FS1.