Heatmap of differential gene expression in Pax5 ^∆OP/∆OP , Pax5 ^∆HD/∆HD , and Pax5 ^{∆OP,HD/∆OP,HD} pro-B cells at higher magnification. (A) The heatmap of the differentially expressed genes, shown in Fig. 2 F, is enlarged to show the expression of the individual genes in Pax5^∆OP/∆OP, Pax5^∆HD/∆HD, and Pax5^{∆OP,HD/∆OP,HD} pro-B cells relative to Pax5^+/+ pro-B cells. For further information, see the legend of Fig. 2 F. (B) Activated genes that were similarly deregulated in Pax5^∆HD/∆HD pro-B cells as well as in Pax5^∆CRD1/− and Pax5^{∆CRD1,2/−} pro-B cells (with the indicated genes belonging to the CRD-regulated gene cluster C (green) or D (orange; Table S2). (C) The genes Cgas, Eef1akmt1, and Hnrnpll (blue) were equally depressed in Pax5^∆OP/∆OP pro-B cells and Pax5^∆CRD1/–, Pax5^∆CRD2/–, and Pax5^{∆CRD1,2/–} pro-B cells (belonging to the CDR-regulated gene cluster D; Fig. 5 D and Fig. S4 D). (D) The genes Chdh, Ptpn3, and Orm2 are derepressed in Pax5^∆OP/∆OP pro-B cells (red), but are not expressed in Pax5^∆CRD1/−, Pax5^∆CRD2/−, and Pax5^{∆CRD1,2/−} pro-B cells as they belonging to the CRD-regulated gene cluster A (Fig. 5 D and Fig. S4 D). The expression of Chdh, Ptpn3, and Orm2 in Pax5^+/+ and Pax5^∆OP/∆OP pro-B cells is shown as mean TPM values of two RNA-seq experiments per genotype. (E) Principal component analysis based on open chromatin data that were generated by ATAC-seq analysis of ex vivo sorted Pax5^+/+, Pax5^{∆CRD1/∆CRD1}, Pax5^{∆CRD1,2/∆CRD1,2} pro-B, and Pax5^∆/∆ (Vav-Cre Pax5^fl/fl) progenitor cells of the bone marrow. CD19^–, CD19⁺, and unfractionated CD19^mix cells were sorted as Lin^–Ly6D⁺B220⁺Kit^hi cells from the bone marrow prior to ATAC-seq analysis.