HDM-induced airway inflammation following influenza A virus infection decreases the frequency of T-bet–expressing NP-specific CD4 ⁺ T _RM in the lung. (A) Schematic representing the experimental timeline. (B) Representative flow cytometry plots of CD4⁺ T cells isolated from the lung at the time point indicated in A. (C) Summary data of the percentage of T-bet⁺ NP-specific cells, geometric mean fluorescence intensity of T-bet in NP-specific cells, and the percentage of ST2⁺ NP-specific cells as represented in B. Data are pooled from eight mice per group from two independent experiments. (D) Representative flow cytometry plots of CD4⁺ T cells isolated from the lung at the time point indicated in A. (E) Summary data of the percentage of GATA-3⁺ NP-specific cells and geometric mean fluorescence intensity of GATA-3 in NP-specific cells as represented in D. Data are pooled from six mice per group from two independent experiments. (F) Representative flow cytometry plots of CD4⁺ T cells isolated from the lung at the time point indicated in A. (G) Summary data of the percentage of Foxp3⁺ NP-specific cells and geometric mean fluorescence intensity of Foxp3 in NP-specific cells as represented in F. Data are pooled from five to seven mice per group from two independent experiments. (H) Schematic of the experimental timeline for data in I and J. (I) Representative flow cytometry plots of CD4⁺ T cells isolated from the lung at experimental day 64. (J) Summary data of the percentage of T-bet⁺ NP-specific cells, the geometric mean fluorescence intensity of T-bet in NP-specific cells, and the percentage of ST2⁺ NP-specific cells as represented in I. Data are pooled from seven mice per group from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Graphs show mean ± SD and data were analyzed by unpaired t test. pfu, plaque-forming units. IAV, influenza A virus. o.p., oropharyngeal. gMFI, geometric mean fluorescence intensity.