spg-7 knockdown enhances the transcription of import machinery components. (a) glp-1(e2141ts) animals were grown at 25°C on bacteria expressing spg-7 dsRNA (diluted to 1:1 ratio with bacteria containing the empty RNAi vector alone) from hatching until the first of adulthood. RNA was isolated on day 1 of adulthood, and qPCR analysis was performed. Expression was normalized against three housekeeping genes and quantified with Student’s t test (two-tailed). The graph is presented as mean ± SD, n = 3. *, P < 0.05; **, P < 0.01.