CD4 T cell–mediated immune responses in CCL19 ^cre and CD25 ^fl/fl CCL19 ^cre mice after EAE induction. (A) Targeting of the IL2rα (CD25) locus. (B) The expression of CD25, CD122, and CD132 on CD31⁻gp38⁺ TRCs from freshly isolated iLNs of CCL19^cre (black bars) or CD25^fl/flCCL19^cre (red bars) mice was measured by flow cytometry (top). The expression of CD25 on CD4⁺ T cells, CD8⁺ T cells, CD11c⁺ DCs, and B220⁺ B cells from spleens of CCL19^cre (black bars) or CD25^fl/flCCL19^cre (red bars) mice was measured by flow cytometry (bottom). Gray histograms represent fluorescence minus one (FMO) control. **, P < 0.01 compared with the CCL19^cre mice. Error bars indicate the mean ± SEM (n = 6). (C) Measurement of cytokine production in culture supernatants from restimulated iLN cells and splenocytes by ELISA. * P < 0.05 compared with the CCL19^cre EAE-induced control group. Error bars indicate the mean ± SEM (n ≥ 6 per group). (D) Representative and compiled data of Foxp3⁺CD25⁺ regulatory T (Treg) cells in CD4⁺ T cells as assessed by flow cytometric analysis of iLNs and spleens. Error bars indicate the mean ± SEM (n ≥ 6 per group). Data are representative of three independent experiments.