Enhanced EAE phenotypes as a result of CD25 deficiency in TRCs. Reconstituted mice were immunized with MOG_35–55, CFA, and pertussis toxin to induce EAE (RAG1^−/−, n = 10; or CD25^−/−RAG1^−/−, n = 6). (A) The mean clinical scores representing disease severity were observed for the indicated times. *, P < 0.05; **, P < 0.01 compared with RAG1-deficient EAE-induced mice. Error bars indicate the mean ± SEM. (B) Disease incidence was assessed at the indicated times. (C and D) IFN-γ⁺ or IL-17A⁺ CD4⁺ T cell populations were analyzed by flow cytometry and quantified in CNS (C) or iLN (D) populations. *, P < 0.05 compared with RAG1-deficient EAE-induced mice. Error bars indicate the mean ± SEM. Data are representative of three independent experiments.