Figure 1.
LN-TRCs express only CD25 and cannot induce IL-2–mediated signaling. (A) Representative flow cytometry sorting strategy for the isolation of TRCs from 5-d cultured LNSCs. LN-TRCs were isolated using a FACS Aria cell sorter. (B) Flow cytometry histograms show the mean fluorescence intensity of IL-2R subunits on TRCs. Gray histograms represent isotype control. (C) Expression of CD25 in WT or CD25-deficient TRCs was examined by Western blot analysis. β-Actin was used as a loading control. (D) Phosphorylation of STAT5 in WT or CD25-deficient TRCs after treatment with rIL-2 (20 ng/ml) at the indicated time points. STAT5 phosphorylation in naive CD4+ T cells is a positive control, and β-actin is a loading control. Data are representative of three independent experiments.

LN-TRCs express only CD25 and cannot induce IL-2–mediated signaling. (A) Representative flow cytometry sorting strategy for the isolation of TRCs from 5-d cultured LNSCs. LN-TRCs were isolated using a FACS Aria cell sorter. (B) Flow cytometry histograms show the mean fluorescence intensity of IL-2R subunits on TRCs. Gray histograms represent isotype control. (C) Expression of CD25 in WT or CD25-deficient TRCs was examined by Western blot analysis. β-Actin was used as a loading control. (D) Phosphorylation of STAT5 in WT or CD25-deficient TRCs after treatment with rIL-2 (20 ng/ml) at the indicated time points. STAT5 phosphorylation in naive CD4+ T cells is a positive control, and β-actin is a loading control. Data are representative of three independent experiments.

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