The sex-specific factors and CA neurons do not mediate the sexually dimorphic cholinergic transmission at NMJs. (A) The table lists the sex-specific factors examined by mutations or drug treatment and their impact on higher aldicarb sensitivity in males. (B–G) Time course analysis of 1.4 mM aldicarb-induced paralysis in hermaphrodites (Herm.) and males with daf-22 (B), daf-12 (C), gon-2 (D), fog-2 (E), spe-9 (F), or glp-1 (G) mutation. (H) Time course analysis of 1.4 mM aldicarb-induced paralysis in hermaphrodites and males with 120 μM FUdR treatment. (I and J) Endogenous acetylcholine transmission was assessed by recording mEPSCs from body-wall muscles of lin-39 mutant hermaphrodites (Herm.) and males. Representative mEPSC traces (I) and the mean mEPSC frequencies (J) are shown. The data for WT are the same as in Fig. 1, H and I. In B–H, for each group, n = 3 biologically independent samples; each sample contains ≥25 animals. Data are presented as mean values ± SEM. Two-way ANOVA comparing all of the time points. In J, data are presented as mean values ± SEM. Ns represent the number of animals tested. One-way ANOVA with post-hoc Bonferroni’s multiple comparisons. ***P < 0.001, n.s. not significant.