Males accumulate relatively more SVs and DCVs at their cholinergic motor neuron terminals. (A and C) Representative micrographs and 3D reconstruction of cholinergic synaptic profiles (A) and GABAergic synaptic profile (C) in hermaphrodites (Herm.) and males (Male). Yellow arrows indicate the dense projection (DP). In the 3D reconstruction, the synaptic vesicles (SVs) are marked as green, the docked synaptic vesicles (DSVs) are marked as red, the dense core vesicles (DCVs) are marked as white, and the DP is marked as yellow. Scale bar, 200 nm. (B and D) The number of cholinergic total SVs, DSVs, and DCVs in a 50 nm cholinergic synaptic profile (B) and GABAergic synaptic profile (D) as well as the synaptic profile area are measured in hermaphrodites (Herm.) and males. In B and D, data are presented as mean values ± SEM. Ns represent the number of synapses analyzed. Two-tailed and unpaired Student’s t test. *P < 0.05, **P < 0.01, n.s. not significant.
Figure 4.

Males accumulate relatively more SVs and DCVs at their cholinergic motor neuron terminals. (A and C) Representative micrographs and 3D reconstruction of cholinergic synaptic profiles (A) and GABAergic synaptic profile (C) in hermaphrodites (Herm.) and males (Male). Yellow arrows indicate the dense projection (DP). In the 3D reconstruction, the synaptic vesicles (SVs) are marked as green, the docked synaptic vesicles (DSVs) are marked as red, the dense core vesicles (DCVs) are marked as white, and the DP is marked as yellow. Scale bar, 200 nm. (B and D) The number of cholinergic total SVs, DSVs, and DCVs in a 50 nm cholinergic synaptic profile (B) and GABAergic synaptic profile (D) as well as the synaptic profile area are measured in hermaphrodites (Herm.) and males. In B and D, data are presented as mean values ± SEM. Ns represent the number of synapses analyzed. Two-tailed and unpaired Student’s t test. *P < 0.05, **P < 0.01, n.s. not significant.

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