The sexually dimorphic cholinergic transmission is not likely mediated by postsynaptic acetylcholine receptors. (A and B) Time course analysis of 1.4 mM aldicarb-induced paralysis in acr-16 (A) and unc-29 (B) mutant hermaphrodites (Herm.) and males (Male). (C–E) Endogenous acetylcholine transmission was assessed by recording mEPSCs from body-wall muscles of wild-type and unc-29 mutant adult hermaphrodites (Herm.) and males (Male). Representative mEPSC traces (C), the mean mEPSC frequencies (D), and the mean mEPSC amplitudes (E) are shown. The data for WT are the same as in Fig. 1, H and I. In A and B, for each group, n = 3 biologically independent samples; each sample contains ≥25 animals. Data are presented as mean values ± SEM. Two-way ANOVA comparing all of the time points. In D and E, data are presented as mean values ± SEM. Ns represent the number of animals tested. One-way ANOVA with post-hoc Bonferroni’s multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. not significant.