The sexually dimorphic synaptic transmission at NMJs arises at the L4 stage and exists in various other natural strains. (A–D) The sexually dimorphic synaptic transmission at NMJs arises at the L4 stage. (A) Schematic illustration of the life cycles of C. elegans and the time when the aldicarb sensitivity assay was applied. (B–D) Time course analysis of 1.4 mM aldicarb-induced paralysis in the L3-staged (B), L4-staged (C), and adult (D) hermaphrodites (Herm.) and males (Male). The data for the adult are the same as in Fig. 1 F. (E–G) The sexually dimorphic synaptic transmission at NMJs exist in various other natural strains. Time course analysis of 1.4 mM aldicarb-induced paralysis in adult hermaphrodites (Herm.) and males (Male) in TR389 (E), AB3 (F), and CB4856 (G) strains. In B–G, for each of the group, n = 3 biologically independent samples; each sample contains ≥25 animals. Data are presented as mean values ± SEM. Two-way ANOVA comparing all of the time points. ***P < 0.001. n.s. not significant.